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Product Name
Anti-DCXR / HCR2 antibody
- Documents
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Description
Rabbit polyclonal to DCXR / HCR2
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human DCXR / HCR2
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Alternative names
dicarbonyl/L-xylulose reductase antibody; DCXR antibody; DCR antibody; HCR2 antibody; HCRII antibody; KIDCR antibody; P34H antibody; SDR20C1 antibody; XR antibody; L-xylulose reductase antibody; carbonyl reductase 2 antibody; carbonyl reductase II antibody; kidney dicarbonyl reductase antibody; short chain dehydrogenase/reductase family 20C antibody; member 1 antibody; sperm surface protein P34H antibody; dicarbonyl L-xylulose reductase antibody; Dcxr antibody; RP23-84C12.19-003 antibody; 0610038K04Rik antibody; 1810027P18Rik antibody; L-xylulose reductase antibody; XR antibody; diacetyl/L-xylulose reductase antibody; XR antibody; DCR antibody; HCR2 antibody; P34H antibody; HCRII antibody; KIDCR antibody; PNTSU antibody; SDR20C1 antibody; 0610038K04Rik antibody; 1810027P18Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human DCXR / HCR2 (rh DCXR / HCR2; Q7Z4W1; Met1-Cys244). DCXR / HCR2 specific IgG was purified by Human DCXR / HCR2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human DCXR / HCR2. The detection limit for Human DCXR / HCR2 is < 0.039 ng/well.
IHC-P: 0.05-0.5 μg/ml
IP: 1-4 μg/mg of lysate
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Validations
DCXR / HCR2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
DCXR / HCR2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
DCXR / HCR2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
DCXR / HCR2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
DCXR / HCR2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
DCXR, also known as HCR2, belongs to the short-chain dehydrogenases/reductases (SDR) family. It is highly expressed in kidney, liver and epididymis. In the epididymis, DCXR is mainly expressed in the proximal and distal sections of the corpus region. HCR2 is weakly or not expressed in brain, lung, heart, spleen and testis. DCXR catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. DCXR participates in the uronate cycle of glucose metabolism. It may play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.
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References
- Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40.
- Pierce SB, et al. (2011) Garrod's fourth inborn error of metabolism solved by the identification of mutations causing pentosuria. Proc Natl Acad Sci. 108(45):18313-7.
- Udeshi ND, et al. (2012) Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Mol Cell Proteomics. 11(5):148-59.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"