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Product Name
Anti-CRK (3H7) Mouse antibody
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Description
CRK (3H7) Mouse monoclonal antibody
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Tested applications
WB
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Species reactivity
Human
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Alternative names
Adapter molecule crk;Avian sarcoma virus CT10 (v crk) oncogene homolog;CRK;CRK isoform 2;CRK isoform II;CRK_HUMAN;CRKII;FLJ38130;OTTHUMP00000115366;OTTHUMP00000198330;p38; Proto oncogene C crk;Proto-oncogene C-crk;v crk avian sarcoma virus CT10 oncogene h antibody
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Isotype
Mouse IgG2b
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Preparation
Antigen: Purified recombinant human CrkII protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
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Validations
Western blot detection of CrkII in Lncap,A431,MCF7 and HT1080 cell lysates using CrkII mouse mAb (1:1000 diluted).Predicted band size: 34kDa.Observed band size: 34kDa.
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Background
Swiss-Prot Acc.P46108.The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"