Anti-CNTN2 antibody

Cat.#: 176597

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Product Information

  • Product Name
    Anti-CNTN2 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to CNTN2
  • Tested applications
    WB, ICC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    AXT antibody; TAX antibody; TAX1 antibody; FAME5 antibody; TAG-1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was recombinant protein within human tag1 aa 40-230.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

    ICC: 1:50-1:200

  • Validations

    Fig1:; Western blot analysis of TAG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: Mouse cerebellum tissue lysate; Lane 2: Human brain tissue lysate; Lane 3: Rat cerebellum tissue lysate

    Fig1:; Western blot analysis of TAG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: Mouse cerebellum tissue lysate; Lane 2: Human brain tissue lysate; Lane 3: Rat cerebellum tissue lysate

    Fig2:; ICC staining of TAG1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    Fig2:; ICC staining of TAG1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Background
  • References
    • von Landenberg N. et. al. Conditional analyses of recurrence and progression in patients with TaG1 non-muscle-invasive bladder cancer. Urol Oncol. 2018 May
    • Zhen YB. et. al. Expression of TAG1/APP signaling pathway in the proliferation and differentiation of glioma stem cells. Oncol Lett. 2017 Aug

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"