Anti-CD96 antibody

Cat.#: 175077

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Product Information

  • Product Name
    Anti-CD96 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to CD96
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Mouse
  • Alternative names
    TACTILE antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    This antigen of this antibody was purified recombinant fragment of human cd96 (aa: extra 321-519) expressed in e. coli.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*PBS with 0.05% sodium azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

    FC: 1:100-1:200

  • Validations

    Fig1: Western blot analysis of CD96 against human CD96 (AA: extra 321-519) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig1: Western blot analysis of CD96 against human CD96 (AA: extra 321-519) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2: Western blot analysis of 175077# against HEK293 (1) and CD96 (AA: extra 321-519)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2: Western blot analysis of 175077# against HEK293 (1) and CD96 (AA: extra 321-519)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig3: Western blot analysis of 175077# againstHela (1), Jurkat (2), MOLT4 (3), Raji (4), HL-60 (5), U937 (6), and C6 (7) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig3: Western blot analysis of 175077# againstHela (1), Jurkat (2), MOLT4 (3), Raji (4), HL-60 (5), U937 (6), and C6 (7) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig4: Flow cytometric analysis of CD96 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody ( 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

    Fig4: Flow cytometric analysis of CD96 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody ( 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

  • Background
  • References
    • Clin Cancer Res. 2016 Nov 1;22(21):5183-5188.
    • Clin Lab. 2015;61(10):1429-34.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"