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Product Name
Anti-CD90/THY-1 antibody
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Description
Rabbit polyclonal to CD90/THY-1
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse THY1 / CD90
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Alternative names
CD90 antibody; CD90 antibody; CDw90 antibody; FLJ33325 antibody; T25 antibody; Thy1 antibody; THY-1 antibody; Thy-1 antibody; Thy1.1 antibody; Thy1.2 antibody; Thy-1.2 antibody; T25 antibody; CD90 antibody; Thy-1 antibody; Thy1.1 antibody; Thy1.2 antibody; Thy-1.2 antibody; CD90 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse THY1 (rM THY1; NP_033408.1; Met 1-Cys 131). THY1 specific IgG was purified by mouse THY1 affinity chromatography
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse THY1. The detection limit for Mouse THY1 is approximately 0.039 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
CD90 / THY-1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse THY1 in rat retina with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to ganglion cells.
CD90 / THY-1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse THY1 in rat kidney with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to renal glomerulus.
CD90 / THY-1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse THY1 in rat brain with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Mouse Thy-1 membrane glycoprotein, also known as Thy-1 antigen, CD90 and THY1, is a cell membrane protein which contains 1 Ig-like V-type (immunoglobulin-like) domain. It is a glycophosphatidylinositol-linked glycoprotein expressed on the surface of neurons, thymocytes, subsets of fibroblasts, endothelial cells, mesangial cells and some hematopoietic cells. It has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells. Thy-1 is evolutionarily conserved, developmentally regulated, and often has dramatic effects on cell phenotype. Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. Thy-1 is an important regulator of cell-cell and cell-matrix interactions, with important roles in nerve regeneration, metastasis, inflammation, and fibrosis.
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References
- Rege TA, et al. (2006) Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis. FASEB J. 20(8): 1045-54.
- Fiegel HC, et al. (2008) Lack of Thy1 (CD90) expression in neuroblastomas is correlated with impaired survival. Pediatr Surg Int. 24(1): 101-5.
- Bradley JE, et al. (2009) Roles and regulation of Thy-1, a context-dependent modulator of cell phenotype. Biofactors. 35(3): 258-65.
- Kisselbach L, et al. (2009) CD90 Expression on human primary cells and elimination of contaminating fibroblasts from cell cultures. Cytotechnology. 59(1): 31-44.
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