Anti-CD123/IL3RA antibody

Cat.#: 106354

Size:

Special Price 193.4 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Anti-CD123/IL3RA antibody
  • Documents
  • Description
    Rabbit monoclonal to CD123/IL3RA
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human IL3RA / CD123
    No cross-reactivity in ELISA with Human IL6R
  • Alternative names
    IL3R antibody; CD123 antibody; IL3RX antibody; IL3RY antibody; IL3RAY antibody; hIL-3Ra antibody; CD123 antibody; SUT-1 antibody; CDw123 antibody; CD123 antibody; CD123 antibody; CDw123 antibody; hIL-3Ra antibody; IL3R antibody; Il3ra antibody; IL3RA antibody; IL3RAY antibody; IL3RX antibody; IL3RY antibody; MGC34174 antibody; RP11-261P4.2 antibody; SUT-1 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained from a rabbit immunized with purified, recombinant Human IL3RA / CD123 (rh L3RA; NP_002174.1; Met 1-Arg 305).
  • Clonality
    Monoclonal
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 20-30 μg/ml

    ELISA: 0.1-0.2 μg/mL

    This antibody can be used at 0.1-0.2 μg/mL μg/ml with the appropriate secondary reagents to detect Human CD123 / IL3RA.

    IP: 1-4 μg/mg of lysate

  • Validations

    IL3RA / CD123 Antibody, Rabbit MAb, Western blot

    IL3RA / CD123 Antibody, Rabbit MAb, Western blot

    IL3RA / CD123 Antibody, Rabbit MAb, Immunoprecipitation

    IL3RA / CD123 Antibody, Rabbit MAb, Immunoprecipitation

  • Background
    Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and / or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.
  • References
    • Del Giudice I, et al. (2004) The diagnostic value of CD123 in B-cell disorders with hairy or villous lymphocytes. Haematologica. 89(3): 303-8.
    • Du X, et al. (2007) New immunotoxins targeting CD123, a stem cell antigen on acute myeloid leukemia cells. J Immunother. 30(6): 607-13.
    • Yue LZ, et al. (2010) Expression of CD123 and CD114 on the bone marrow cells of patients with myelodysplastic syndrome. Chin Med J (Engl). 123(15): 2034-2037.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"