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Product Name
Anti-Cathepsin A/CTSA antibody
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Description
Rabbit polyclonal to Cathepsin A/CTSA
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Tested applications
ELISA, WB, IP
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Species reactivity
Mouse Cathepsin A / CTSA
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Alternative names
AU019505 antibody; Cathepsin A antibody; Cathepsin A antibody; Ctsa antibody; CTSA antibody; GLB2 antibody; GSL antibody; NGBE antibody; PPCA antibody; PPCA antibody; Ppgb antibody; PPGB antibody; RP23-61O3.6 antibody; RP3-337O18.1 antibody; GSL antibody; GLB2 antibody; NGBE antibody; PPCA antibody; PPGB antibody; PPCA antibody; Ppgb antibody; AU019505 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse Cathepsin A / CTSA (rh Cathepsin A / CTSA; P16675-1; Met1-Tyr474). Cathepsin A / CTSA specific IgG was purified by Mouse Cathepsin A / CTSA affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse Cathepsin A / CTSA. The detection limit for Mouse Cathepsin A / CTSA is < 0.039 ng/well.
IP: 4-6 μg/mg of lysate
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Validations
Cathepsin A / CTSA Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Cathepsin A / CTSA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with beta-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation. Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. It was recently identified in human platelets as deamidase. In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions. It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases. Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase. Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation.
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References
- Hiraiwa M. (1999) Cathepsin A/protective protein: an unusual lysosomal multifunctional protein. Cell Mol Life Sci. 56(11-12): 894-907.
- Yoshida T, et al. (2006) Comparative analysis of binding energy of chymostatin with human cathepsin A and its homologous proteins by molecular orbital calculation. J Chem Inf Model. 46(5): 2093-103.
- Seyrantepe V, et al. (2008) Enzymatic activity of lysosomal carboxypeptidase (cathepsin) A is required for proper elastic fiber formation and inactivation of endothelin-1. Circulation. 117(15): 1973-81.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"