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Product Name
Anti-CARKL / SHPK antibody
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Description
Rabbit polyclonal to CARKL / SHPK
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human CARKL / SHPK
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Alternative names
Sedoheptulokinase antibody; SHPK antibody; CARKL antibody; SHK antibody; carbohydrate kinase-like protein antibody; Sedoheptulokinase antibody; Shpk antibody; RP23-214D6.1 antibody; 4930431K22Rik antibody; AI194947 antibody; AW260459 antibody; Carkl antibody; SHK antibody; carbohydrate kinase-like protein antibody; SHK antibody; CARKL antibody; Carkl antibody; AI194947 antibody; AW260459 antibody; 4930431K22Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human CARKL / SHPK (rh CARKL / SHPK; NP_037408.2; Ala2-Ser478). CARKL / SHPK specific IgG was purified by Human CARKL / SHPK affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CARKL / SHPK. The detection limit for Human CARKL / SHPK is < 0.039 ng/well.
IHC-P: 0.1-2 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
CARKL / SHPK Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human SHPK in human liver with rabbit polyclonal antibody (0.3 µg/mL, formalin-fixed paraffin embedded sections).
CARKL / SHPK Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
CARKL / SHPK Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
CARKL / SHPK Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human SHPK in human pancreas with rabbit polyclonal antibody (0.3 µg/mL, formalin-fixed paraffin embedded sections).
CARKL / SHPK Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human SHPK in human kidney with rabbit polyclonal antibody (0.3 µg/mL, formalin-fixed paraffin embedded sections).
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Background
CARKL, also known as SHPK, is a nonprotein kinase of glucose metabolism. CARKL has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. CARKL catalyzes an orphan reaction in the pentose phosphate pathway, refocusing cellular metabolism to a high-redox state upon physiological or artificial downregulation. CARKL-dependent metabolic reprogramming is required for proper M1- and M2-like macrophage polarization and uncover a rate-limiting requirement for appropriate glucose flux in macrophage polarization.
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References
- Haschemi A. et al., 2012, Cell Metab. 15 (6): 813-26.
- Udeshi ND. et al., 2012, Mol Cell Proteomics. 11 (5): 148-59.
- Wamelink MM. et al., 2008, Hum Mutat. 29 (4): 532-6.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"