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Product Name
Anti-Carbonic Anhydrase XIII/CA13 antibody
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Description
Rabbit polyclonal to Carbonic Anhydrase XIII/CA13
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Tested applications
ELISA, WB, IP
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Species reactivity
Human Carbonic Anhydrase XIII / CA13
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Alternative names
2310075C21Rik antibody; CA13 antibody; Ca13 antibody; Car13 antibody; Carbonic Anhydrase XIII antibody; Carbonic Anhydrase XIII antibody; CAXIII antibody; CA-XIII antibody; CA-XIII antibody; FLJ37995 antibody; MGC59868 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase XIII / CA13 (rh Carbonic Anhydrase XIII / CA13; NP_940986.1; Met 1-His 262). Carbonic Anhydrase XIII / CA13 specific IgG was purified by Human Carbonic Anhydrase XIII / CA13 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 1-2 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CA13. The detection limit for Human CA13 is approximately 0.00975 ng/well.
IP: 4-6 μg/mg of lysate
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Validations
Carbonic Anhydrase XIII / CA13 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Carbonic Anhydrase XIII / CA13 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. The CAXIII is a member of the CA family, which owns a globular molecule with high structural similarity to cytosolic isozymes, CAI, II, and III. Recombinant mouse CAXIII showed catalytic activity similar to those of mitochondrial CAV and cytosolic CAI. In human tissues, CAXIII expression was identified in the thymus, small intestine, spleen, prostate, ovary, colon, and testis. In mouse, positive tissues included the spleen, lung, kidney, heart, brain, skeletal muscle, and testis. In conclusion, the predicted amino acid sequence, structural model, distribution, and activity data suggest that CAXIII represents a novel enzyme, which may play important physiological roles in several organs.
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References
- Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
- Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1)：1-20.
- Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"