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Product Name
Anti-BLMH/Bleomycin Hydrolase antibody
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Description
Rabbit polyclonal to BLMH/Bleomycin Hydrolase
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Tested applications
ELISA, WB, IP
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Species reactivity
Mouse BLMH / Bleomycin Hydrolase
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Alternative names
AI035728 antibody; BH antibody; Bleomycin Hydrolase antibody; Blmh antibody; BMH antibody; RP23-100P23.3 antibody; Bh antibody; Bleomycin Hydrolase antibody; BLMH antibody; Bmh antibody; MGC36899 antibody; MGC37104 antibody; MGC39026 antibody; BH antibody; BMH antibody; Bh antibody; Bmh antibody; AI035728 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse BLMH / Bleomycin Hydrolase (rM BLMH / Bleomycin Hydrolase; NP_848760.1; Asn 2-Glu 455). BLMH / Bleomycin Hydrolase specific IgG was purified by Mouse BLMH / Bleomycin Hydrolase affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse BLMH. The detection limit for Mouse BLMH is approximately 0.00245 ng/well.
IP: 1-4 μg/mg of lysate
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Validations
BLMH / Bleomycin Hydrolase Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
BLMH / Bleomycin Hydrolase Antibody, Rabbit PAb, Antigen Affinity Purified
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Background
The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.
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References
- Takeda A, et al. (1996) Cloning and Analysis of cDNA Encoding Rat Bleomycin Hydrolase, a DNA-Binding Cysteine Protease. J Biochem. 120 (2): 353-9.
- Lefterov IM, et al. (2000) Human bleomycin hydrolase regulates the secretion of amyloid precursor protein. The FASEB Journal. 14(12): 1837-47.
- Brmme D, et al. (1996) Human Bleomycin Hydrolase: Molecular Cloning, Sequencing, Functional Expression, and Enzymatic Characterization. Biochemistry. 35 (21): 6706-14.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"