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Product Name
ANO2 antibody
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Description
ANO2 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, HeLa cells, human brain tissue, mouse eye tissue. Positive IP detected in mouse brain tissue. Positive IF detected in HepG2 cells, Hela cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 114kd
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Tested applications
ELISA, WB, IF, IP, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ANO2 antibody; anOCtamin 2 antibody; C12orf3 antibody; DKFZp434P102 antibody; TMEM16B antibody; Transmembrane protein 16B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_020373). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108101(ANO2 antibody) at dilution of 1:500
IP Result of anti-ANO2 (IP:Catalog No:108101, 4ug; Detection:Catalog No:108101 1:300) with mouse brain tissue lysate 10000ug.
Immunofluorescent analysis of HepG2 cells, using ANO2 antibody Catalog No:108101 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:108101(ANO2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"