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Product Name
ANKS1B antibody
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Description
ANKS1B Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in A549 cells, HeLa cells, mouse testis tissue. Positive IHC detected in human testis tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 65-70 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
AIDA antibody; AIDA 1 antibody; ANKS1B antibody; ANKS2 antibody; cajalin 2 antibody; E2A PBX1 assOCiated protein antibody; EB 1 antibody; EB1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ANKS1B recombinant protein (Accession Number: NM_181670). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:108085(ANKS1B Antibody) at dilution of 1:1000
IP Result of anti-ANKS1B (IP:Catalog No:108085, 4ug; Detection:Catalog No:108085 1:1000) with HeLa cells lysate 3200ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:108085(ANKS1B Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:108085(ANKS1B Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:108085(ANKS1B Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
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Background
ANKS1B also named as AIDA or cajalin 2 is a amino acid protein, which contains 7 ANK repeats, 1 PID domain and 2 SAM domians. ANKS1B localizes in cytoplasm and is highly expressed in brain and testis. ANKS1B exists as many alternatively spliced isoforms. It interacts with AbPP to play a role in brain development. AIDA-1 also interacts with coilin in Cajal bodies to regulate pre-mRNA splicing.
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