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Product Name
Amylase, alpha antibody
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Description
Amylase, alpha Rabbit Polyclonal antibody. Positive IHC detected in human pancreas tissue. Positive WB detected in human liver tissue, mouse pancreas tissue. Observed molecular weight by Western-blot: 58 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
AMY2A antibody; Amylase antibody; alpha antibody; Pancreatic alpha amylase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Amylase, alpha recombinant protein (Accession Number: NM_000699). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:108014(AMY2A antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:108014(AMY2A antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:108014(AMY2A antibody) at dilution of 1:100 (under 40x lens)
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Background
The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258).AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956). 15845-1-AP can recognize both AMY1 and AMY2.
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