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Product Name
Amylase, alpha antibody
- Documents
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Description
Amylase, alpha Mouse Monoclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive IF detected in BxPC-3 cells. Positive WB detected in human saliva. Observed molecular weight by Western-blot: 58 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
AMY2A antibody; Amylase antibody; alpha antibody; Pancreatic alpha amylase antibody
- Immunogen
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of Amylase, alpha recombinant protein (Accession Number: NM_000699). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human saliva were subjected to SDS PAGE followed by western blot with Catalog No:107583(AMY2A antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:107583(AMY2A Antibody) at dilution of 1:50(under 10x lens)
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:107583(AMY2A Antibody) at dilution of 1:50(under 40x lens)
Immunofluorescent analysis of BxPC-3 cells using Catalog No:107583(AMY2A Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258). It has a hydrolase activity acting on glycosyl bonds. AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956).This antibody can recognize amylase alpha.
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References
- Kuroda Y, Hayashi S, Hagio S, Abe M, Furukawa S, Nakae D. Pancreatic Ductal Adenocarcinoma in a Wistar Hannover GALAS Rat. Journal of toxicologic pathology. 27(2):147-51. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"