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Product Name
AMPK alpha 2 antibody
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Description
AMPK alpha 2 Rabbit Polyclonal antibody. Positive IHC detected in human skeletal muscle tissue, human pancreas tissue. Positive IF detected in MCF-7 cells. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in human skeletal muscle tissue, HeLa cells, MCF7 cells, rat ovary tissue, SKOV-3 cells. Observed molecular weight by Western-blot: 62 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AMPK antibody; AMPK alpha 2 antibody; AMPK subunit alpha 2 antibody; AMPK2 antibody; PRKAA antibody; PRKAA2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AMPK alpha 2 recombinant protein (Accession Number: NM_006252). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:108007(PRKAA2 antibody) at dilution of 1:500
Immunofluorescent analysis of MCF-7 cells, using PRKAA2 antibody Catalog No:108007 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PRKAA2 (IP:Catalog No:108007, 4ug; Detection:Catalog No:108007 1:1000) with mouse skeletal muscle tissue lysate 3600ug.
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:108007(PRKAA2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:108007(PRKAA2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
PRKAA2(protein kinase, AMP-activated, alpha 2 catalytic subunit), also named as AMPKA2, AMPK, PRKAA, AMPK2, belonogs to the CAMK Ser/Thr protein kinase family and SNF1 subfamily. PRKAA2 is an αβγ heterotrimer that is activated by low cellular energy status, such as decreases in both the ATP/AMP ratio and the phosphocreatine content and it is a glycogen synthase kinase, phosphorylating Ser7 at the NH2 terminus, which decreases glycogen synthase activity(PMID:14532170). The protein can be ubiquitinated(PMID:21224036).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"