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Product Name
AMOT antibody
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Description
AMOT Mouse Monoclonal antibody. Positive WB detected in SGC-7901 cells. Positive IHC detected in human placenta tissue, human skeletal muscle tissue. Observed molecular weight by Western-blot: 130 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human; other species not tested.
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Alternative names
AMOT antibody; angiomotin antibody; KIAA1071 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of peptide (Accession Number: NM_001113490). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human placenta using Catalog No:107582(AMOT antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:107582(AMOT antibody) at dilution of 1:100 (under 40x lens)
SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:107582(AMOT Antibody) at dilution of 1:2000
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Background
predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Most abundant expression was found in placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). This antibody recognizes p130 only.
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