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Product Name
AMFR antibody
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Description
AMFR Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells. Positive IP detected in HepG2 cells. Positive IHC detected in human ovary tissue, human heart tissue, human kidney tissue, human liver tissue, human placenta tissue, human skin tissue, human testis tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 73 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AMF receptor antibody; isoform 2 antibody; AMFR antibody; GP78 antibody; RING finger protein 45 antibody; RNF45 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AMFR recombinant protein (Accession Number: NM_001144). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107990(AMFR antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human ovary using Catalog No:107990(AMFR antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary using Catalog No:107990(AMFR antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using AMFR antibody Catalog No:107990 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-AMFR (IP:Catalog No:107990, 3ug; Detection:Catalog No:107990 1:800) with HepG2 cells lysate 2400ug.
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Background
AMFR(Autocrine motility factor receptor) is also named as RNF45,gp78.It is the receptor of Autocrine motility factor(AMF),which is a protein secreted by tumor cells that stimulates tumor motility. AMFR has a potential N-glycosylation site, and several potential O-glycosylation sites(PMID:10456327).In normal fibroblasts,the expression of AMFR appeared to be regulated by cell-cell contact(PMID:7626106).
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References
- Wang Q, Liu X, Cui Y. The E3 ubiquitin ligase AMFR and INSIG1 bridge the activation of TBK1 kinase by modifying the adaptor STING. Immunity. 41(6):919-33. 2014.
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