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Product Name
Alpha galactosidase A antibody
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Description
Alpha galactosidase A Mouse Monoclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells, human kidney tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human liver tissue. Observed molecular weight by Western-blot: 49 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
Alpha galactosidase A antibody; galactosidase antibody; alpha antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of Alpha galactosidase A recombinant protein (Accession Number: NM_000169). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107577(GLA antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:107577(GLA Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:107577(GLA Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:107577(GLA Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG
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Background
GLA, also named as Melibiase, Agalsidas and Alpha-galactosidase A, Belongs to the glycosyl hydrolase 27 family. It hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"