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Product Name
ALKBH7 antibody
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Description
ALKBH7 Rabbit Polyclonal antibody. Positive WB detected in human testis tissue. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 25kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ABH7 antibody; ALKBH7 antibody; SPATA11 antibody; UNQ6002 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ALKBH7 recombinant protein (Accession Number: NM_032306). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:107859(ALKBH7 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:107859(ALKBH7 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:107859(ALKBH7 Antibody) at dilution of 1:50 (under 40x lens)
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Background
ALKBH7(Alkylated DNA repair protein alkB homolog 7) is also named as ABH7, SPATA11 and belongs to the alkB family. This protein may be a dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron. Quantitative real-time PCR detectes variable ALKBH7 expression in all 16 tissues examined, with highest expression in pancreas, followed by spleen, prostate, ovary, and placenta(PMID:17979886). The gene encodes a 25 kDa protein with a 20 amino acid signal peptide.
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