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Product Name
Aldolase C antibody
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Description
Aldolase C Mouse Monoclonal antibody. Positive WB detected in human heart tissue, human brain tissue. Positive IHC detected in human liver cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 39 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
ALDOC antibody; Aldolase C antibody; Brain type aldolase antibody; Fructose bisphosphate aldolase C antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Aldolase C recombinant protein (Accession Number: NM_005165). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:5000-1:50000
IHC: 1:50-1:500
IF: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:107571(ALDoc antibody) at dilution of 1:10000
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:107571(ALDoc Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:107571(ALDoc Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of HepG2 cells using Catalog No:107571(ALDoc Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG
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Background
Fructose-bisphosphate aldolase C (ALDoc) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDoc in signal transduction. Meanwhile, the protein expression of ALDoc has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).
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