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Product Name
ALDH3A1 antibody
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Description
ALDH3A1 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Aldehyde dehydrogenase 3 antibody; ALDH3 antibody; ALDH3A1 antibody; ALDHIII antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ALDH3A1 recombinant protein (Accession Number: NM_000691). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107967(ALDH3A1 antibody) at dilution of 1:2000
Immunofluorescent analysis of MCF-7 cells, using ALDH3A1 antibody Catalog No:107967 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-ALDH3A1 (IP:Catalog No:107967, 4ug; Detection:Catalog No:107967 1:1000) with HEK-293 cells lysate 3200ug.
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Background
ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).
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References
- Ma J, Zhou D, Fan M. Keratocytes create stromal spaces to promote corneal neovascularization via MMP13 expression. Investigative ophthalmology & visual science. 55(10):6691-703. 2014.
- Agyeman AS, Chaerkady R, Shaw PG. Transcriptomic and proteomic profiling of KEAP1 disrupted and sulforaphane-treated human breast epithelial cells reveals common expression profiles. Breast cancer research and treatment. 132(1):175-87. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"