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Product Name
ALDH1B1 antibody
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Description
ALDH1B1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue. Positive IP detected in mouse brain tissue. Positive WB detected in HepG2 cells, mouse brain tissue. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Aldehyde dehydrogenase 5 antibody; ALDH1B1 antibody; ALDH5 antibody; ALDHX antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ALDH1B1 recombinant protein (Accession Number: NM_000692). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107963(ALDH1B1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:107963(ALDH1B1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:107963(ALDH1B1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ALDH1B1 (IP:Catalog No:107963, 4ug; Detection:Catalog No:107963 1:500) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:107963(ALDH1B1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112).
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References
- Oberbeck N, Langevin F, King G, de Wind N, Crossan GP, Patel KJ. Maternal aldehyde elimination during pregnancy preserves the fetal genome. Molecular cell. 55(6):807-17. 2014.
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