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Product Name
ALDH1B1 antibody
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Description
ALDH1B1 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human testis tissue, human liver tissue. Positive WB detected in HeLa cells, HepG2 cells, mouse brain tissue, mouse testis tissue, rat brain tissue. Observed molecular weight by Western-blot: 55-58 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
Aldehyde dehydrogenase 5 antibody; ALDH1B1 antibody; ALDH5 antibody; ALDHX antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ALDH1B1 recombinant protein (Accession Number: NM_000692). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107962(ALDH1B1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis slide using Catalog No:107962(ALDH1B1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human testis slide using Catalog No:107962(ALDH1B1 Antibody) at dilution of 1:50
Immunofluorescent analysis of MCF-7 cells, using ALDH1B1 antibody Catalog No:107962 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112). This antibody is specific to ALDH1B1.
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