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Product Name
ADHFE1 antibody
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Description
ADHFE1 Rabbit Polyclonal antibody. Positive WB detected in rat kidney tissue, mouse heart tissue. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 50 kDa,45 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ADH8 antibody; ADHFE1 antibody; FLJ32430 antibody; HMFT2263 antibody; HOT antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ADHFE1 recombinant protein (Accession Number: BC064634). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
rat kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:107791(ADHFE1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:107791(ADHFE1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107791(ADHFE1 antibody) at dilution of 1:50 (under 40x lens)
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Background
The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues. It belongs to the iron-containing alcohol dehydrogenase family. ADHFE1 has 4 isoforms produced by alternative splicing with the MW of 50, 45, 32, 27 kDa.
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