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Product Name
Adenosine A1 Receptor antibody
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Description
Adenosine A1 Receptor Rabbit Polyclonal antibody. Positive FC detected in SH-SY5Y cells. Positive IHC detected in mouse brain tissue, mouse cerebellum tissue. Positive WB detected in mouse eye tissue. Observed molecular weight by Western-blot: 39 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
adenosine A1 receptor antibody; Adenosine receptor A1 antibody; ADORA1 antibody; RDC7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Adenosine A1 Receptor recombinant protein (Accession Number: NM_000674). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:50-1:500
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Validations
mouse eye tissue were subjected to SDS PAGE followed by western blot with Catalog No:107783(ADORA1 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:107783(ADORA1 Antibody) at dilution of 1:100 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:107783(ADORA1 Antibody) at dilution of 1:100 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
1X10^6 SH-SY5Y cells were stained with 0.2ug ADORA1 antibody (Catalog No:107783, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
ADORA1 represents adenosine A1 receptor
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