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Product Name
ADAR1 antibody
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Description
ADAR1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human gliomas tissue. Positive WB detected in HeLa cells. Positive IP detected in Y79 cells. Observed molecular weight by Western-blot: 110 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human,Mouse,Rat,Pig; other species not tested.
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Alternative names
ADAR antibody; ADAR1 antibody; adenosine deaminase antibody; RNA antibody; DRADA antibody; DSH antibody; DSRAD antibody; G1P1 antibody; IFI 4 antibody; IFI4 antibody; Interferon inducible protein 4 antibody; K88dsRBP antibody; p136 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ADAR1 recombinant protein (Accession Number: NM_001111). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107774(ADAR1 Antibody) at dilution of 1:1000
IP Result of anti-ADAR1 (IP:Catalog No:107774, 4ug; Detection:Catalog No:107774 1:500) with Y79 cells lysate 3000ug.
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:107774(ADAR1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:107774(ADAR1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using ADAR antibody Catalog No:107774 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an interferon (IFN) inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH).ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities(PMID:17428802).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"