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Product Name
ADAM28 antibody
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Description
ADAM28 Rabbit Polyclonal antibody. Positive IP detected in mouse lung tissue. Positive WB detected in mouse lung tissue, BxPC-3 cells, mouse colon tissue, mouse liver tissue, mouse small intestine tissue, mouse spleen tissue, PC-13 cells. Observed molecular weight by Western-blot: 35 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ADAM 28 antibody; ADAM28 antibody; eMDC II antibody; eMDCII antibody; MDC L antibody; MDC Lm antibody; MDC Ls antibody; MDCL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ADAM28 recombinant protein (Accession Number: NM_014265). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:107766(ADAM28 antibody) at dilution of 1:800
IP Result of anti-ADAM28 (IP:Catalog No:107766, 4ug; Detection:Catalog No:107766 1:800) with mouse lung tissue lysate 3600ug.
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Background
ADAM28 belongs to a family of cell surface and secreted glycoproteins that possess both proteolytic and adhesive properties. ADAM28 is predominantly expressed by carcinoma cells in the breast carcinoma tissues as protein bands of 42 kDa and 55/57 kDa, which correspond to the active forms of ADAM28s and ADAM28m, respectively.In human non-small cell lung carcinomas and breast carcinomas, ADAM28 is overexpressed predominantly by carcinoma cells, and the expression correlates with carcinoma cell proliferation and lymph node metastasis(PMID:19601836).
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