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Product Name
ACTN2 antibody
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Description
ACTN2 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, mouse lung tissue, mouse skeletal muscle tissue. Positive IP detected in HeLa cells. Positive IHC detected in human heart tissue. Positive IF detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 103 kDa
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Tested applications
ELISA, WB, IP, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
actinin antibody; alpha 2 antibody; ACTN2 antibody; Alpha actinin 2 antibody; CMD1AA antibody; F actin cross linking protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ACTN2 recombinant protein (Accession Number: NM_001103). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107710(ACTN2 antibody) at dilution of 1:400
IP Result of anti-ACTN2 (IP:Catalog No:107710, 4ug; Detection:Catalog No:107710 1:1000) with HeLa cells lysate 1080ug.
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:107710(ACTN2 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:107710(ACTN2 Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (-20oc Ethanol) fixed NIH/3T3 cells using Catalog No:107710(ACTN2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ACTN2 represents actinin, alpha 2
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