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Product Name
ACSL3 antibody
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Description
ACSL3 Rabbit Polyclonal antibody. Positive IF detected in HepG2. Positive WB detected in mouse kidney tissue, HEK-293 cells. Observed molecular weight by Western-blot: 70-80kd
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Tested applications
ELISA, WB, IF
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Species reactivity
human, mouse; other species not tested.
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Alternative names
ACSL3 antibody; LACS3 antibody; ACS3 antibody; FACL3 antibody; LACS 3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_004457). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:107696(ACSL3 antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells, using ACSL3 antibody Catalog No:107696 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.
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