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Product Name
ACAT1 antibody
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Description
ACAT1 Rabbit Polyclonal antibody. Positive WB detected in human skeletal muscle tissue, human heart tissue. Positive IP detected in mouse heart tissue. Positive IF detected in Hela cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 45kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ACAT antibody; ACAT1 antibody; Acetoacetyl CoA thiolase antibody; MAT antibody; THIL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ACAT1 recombinant protein (Accession Number: BC010942). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:107727(ACAT1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:107727(ACAT1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107727(ACAT1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using ACAT1 antibody Catalog No:107727 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-ACAT1 (IP:Catalog No:107727, 4ug; Detection:Catalog No:107727 1:500) with mouse heart tissue lysate 4400ug.
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Background
ACAT1 represents acetyl-Coenzyme A acetyltransferase 1
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References
- Yao J, Chen S, Mao Z, Cadenas E, Brinton RD. 2-Deoxy-D-glucose treatment induces ketogenesis, sustains mitochondrial function, and reduces pathology in female mouse model of Alzheimer's disease. PloS one. 6(7):e21788. 2011.
- Peralta S, Torraco A, Wenz T, Garcia S, Diaz F, Moraes CT. Partial complex I deficiency due to the CNS conditional ablation of Ndufa5 results in a mild chronic encephalopathy but no increase in oxidative damage. Human molecular genetics. 23(6):1399-412. 2014.
- Hao S, Ji J, Zhao H. Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation through both ROS Scavenging and Inhibition of Cholesterol Influx in RAW264.7 Cells. Molecules (Basel, Switzerland). 20(12):21287-97. 2015.
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