Product Name

ABHD5 antibody

Documents

Description

ABHD5 Rabbit Polyclonal antibody. Positive IHC detected in human skeletal muscle tissue, human liver tissue. Positive IF detected in MCF-7 cells. Positive WB detected in Jurkat cells, human brain tissue, human cerebellum tissue, mouse heart tissue. Observed molecular weight by Western-blot: 39 kDa

Tested applications

ELISA, WB, IF, IHC

Species reactivity

Human, Mouse; other species not tested.

Alternative names

ABHD5 antibody; CDS antibody; CGI58 antibody; IECN2 antibody; NCIE2 antibody

Immunogen

Human ABHD5 Recombinant protein (GST tag)

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of ABHD5 recombinant protein (Accession Number: NM_016006). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:107815(ABHD5 antibody) at dilution of 1:500

Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:107815(ABHD5 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:107815(ABHD5 antibody) at dilution of 1:50 (under 40x lens)

Immunofluorescent analysis of MCF-7 cells, using ABHD5 antibody Catalog No:107815 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Background

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).

References

Granneman JG, Moore HP, Mottillo EP, Zhu Z. Functional interactions between Mldp (LSDP5) and Abhd5 in the control of intracellular lipid accumulation. The Journal of biological chemistry. 284(5):3049-57. 2009.
Yang X, Zhang X, Heckmann BL, Lu X, Liu J. Relative contribution of adipose triglyceride lipase and hormone-sensitive lipase to tumor necrosis factor-α (TNF-α)-induced lipolysis in adipocytes. The Journal of biological chemistry. 286(47):40477-85. 2011.
Yang X, Heckmann BL, Zhang X, Smas CM, Liu J. Distinct mechanisms regulate ATGL-mediated adipocyte lipolysis by lipid droplet coat proteins. Molecular endocrinology (Baltimore, Md.). 27(1):116-26. 2013.
Olzmann JA, Richter CM, Kopito RR. Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover. Proceedings of the National Academy of Sciences of the United States of America. 110(4):1345-50. 2013.
Ding SY, Lee MJ, Summer R, Liu L, Fried SK, Pilch PF. Pleiotropic effects of cavin-1 deficiency on lipid metabolism. The Journal of biological chemistry. 289(12):8473-83. 2014.
Patel S, Yang W, Kozusko K, Saudek V, Savage DB. Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis. Proceedings of the National Academy of Sciences of the United States of America. 111(25):9163-8. 2014.
Zhang X, Heckmann BL, Xie X, Saarinen AM, Liu J. Regulation of FSP27 protein stability by AMPK and HSC70. American journal of physiology. Endocrinology and metabolism. 307(11):E1047-56. 2014.

ABHD5 antibody

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