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Product Name
AARS2 antibody
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Description
AARS2 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells. Positive IP detected in K-562 cells. Positive IHC detected in human lung cancer tissue. Observed molecular weight by Western-blot: 105-115 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
AARS2 antibody; AARSL antibody; Alanine tRNA ligase antibody; Alanyl tRNA synthetase like antibody; AlaRS antibody; bA444E17.1 antibody; KIAA1270 antibody; MT ALARS antibody; MTALARS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AARS2 recombinant protein (Accession Number: NM_020745). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107675(AARS2 antibody) at dilution of 1:3000
Immunohistochemistry of paraffin-embedded human lung cancer slide using Catalog No:107675(AARS2 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human lung cancer slide using Catalog No:107675(AARS2 Antibody) at dilution of 1:50
IP Result of anti-AARS2 (IP:Catalog No:107675, 4ug; Detection:Catalog No:107675 1:1000) with K-562 cells lysate 3600ug.
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Background
AARS2(Alanine--tRNA ligase, mitochondrial) is also named as KIAA1270, AARSL, bA444E17.1, AlaRS, AARSL, COXPD8, MTALARS, MT-ALARS and belongs to the class-II aminoacyl-tRNA synthetase family. It catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). It also edits incorrectly charged tRNA(Ala) via its editing domain. The full length 107 kDa protein has a transit peptide with 23 amino acids.
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