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Product Name
AACS antibody
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Description
AACS Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive WB detected in mouse heart tissue, mouse liver tissue. Observed molecular weight by Western-blot: 67 kDa, 30 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AACS antibody; acetoacetyl CoA synthetase antibody; ACSF1 antibody; FLJ12389 antibody; FLJ41251 antibody; Protein sur 5 homolog antibody; SUR 5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AACS recombinant protein (Accession Number: NM_023928). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: 1:20-1:200
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:107669(AACS Antibody) at dilution of 1:300
Immunofluorescent analysis of MCF-7 cells using Catalog No:107669(AACS Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
AACS(acetoacetyl-CoA synthetase) is also named as ACSF1 and belonogs to the ATP-dependent AMP-binding enzyme family. In mammals, AACS is present in the cytosolic fractions of various tissues, especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids. These results indicate that AACS may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis. It has 3 isoforms produced by alternative splicing.
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