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Product Name
A1BG antibody
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Description
A1BG Rabbit Polyclonal antibody. Positive WB detected in human plasma tissue, BxPC-3 cells, MCF7 cells, mouse kidney tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human hepat℃irrhosis tissue. Observed molecular weight by Western-blot: 65 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
A1B antibody; A1BG antibody; ABG antibody; alpha 1 B glycoprotein antibody; Alpha 1B glycoprotein antibody; DKFZp686F0970 antibody; GAB antibody; HYST2477 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of A1BG recombinant protein (Accession Number: BC035719). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:2000-1:20000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human plasma tissue were subjected to SDS PAGE followed by western blot with Catalog No:107664(A1BG Antibody) at dilution of 1:5000
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:107664(A1BG antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:107664(A1BG antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:107664(A1BG Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
A1BG (alpha-1B-glycoprotein) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).
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