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Product Name
5 Lipoxygenase antibody
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Description
5 Lipoxygenase Rabbit Polyclonal antibody. Positive WB detected in HL-60 cells, DU 145 cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: ~70 kDa
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Tested applications
ELISA, IF, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
5 Lipoxygenase antibody; 5 LO antibody; 5 LOX antibody; 5LPG antibody; ALOX5 antibody; LOG5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Recombinant Protein (Accession Number: NM_000698). Purification method: Protein A purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
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Validations
Immunofluorescent analysis of Hela cells, using ALOX5 antibody Catalog No:107662 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:107662(ALOX5 Antibody) at dilution of 1:600
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Background
ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).
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References
- Sarveswaran S, Chakraborty D, Chitale D, Sears R, Ghosh J. Inhibition of 5-lipoxygenase selectively triggers disruption of c-Myc signaling in prostate cancer cells. The Journal of biological chemistry. 290(8):4994-5006. 2015.
- Sarveswaran S, Ghosh R, Morisetty S, Ghosh J. MK591, a second generation leukotriene biosynthesis inhibitor, prevents invasion and induces apoptosis in the bone-invading C4-2B human prostate cancer cells: implications for the treatment of castration-resistant, bone-metastatic prostate cancer. PloS one. 10(4):e0122805. 2015.
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