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Product Name
14-3-3 GAMMA-Specific antibody
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Description
14-3-3 GAMMA-Specific Rabbit Polyclonal antibody. Positive IF detected in MDCK cells. Positive WB detected in mouse IMCD3 cells (RNAi), A431 cells, human brain tissue, Jurkat cells, K-562 cells, NIH/3T3 cells, U-937 cells. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 28-33 kDa
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Tested applications
ELISA, IP, WB, IF
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Species reactivity
Human,Mouse,Rat, Dog; other species not tested.
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Alternative names
14 3 3 protein gamma antibody; 14 3 3GAMMA antibody; 14-3-3 antibody; 14-3-3 GAMMA antibody; KCIP 1 antibody; YWHAG antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of 14-3-3 GAMMA-Specific recombinant protein (Accession Number: NM_012479). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:107660(14 3 3GAMMA Antibody) at dilution of 1:600
RNAi result of Catalog No:107660(anti-14-3-3G) in Mouse IMCD3 cells From Dr.Seongjin Seo.
IP Result of anti-14 3 3GAMMA (IP:Catalog No:107660, 3ug; Detection:Catalog No:107660 1:800) with mouse brain tissue lysate 8000ug.
Immunofluorescent analysis of MDCK cells using Catalog No:107660(14 3 3GAMMA Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
14-3-3 gamma (also known as YWHAG) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody specifically recognizes gamma isoform of 14-3-3.
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References
- Zeng S, Zhang H, Ding Z. Proteome analysis of porcine epidemic diarrhea virus (PEDV)-infected Vero cells. Proteomics. 15(11):1819-28. 2015.
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